|Location||Call number||Status||Date due|
|Research and Special Collections||RM 666.H33O9||Not for loan|
Thesis (Ph.D.) Kenyatta University, 1997.
Includes bibl. refs.
Herbal medicine is practiced by many countries of the world inlcuding Kenya, but scientific data to suppoprt the efficacy of herbal preparations has been lacking. Sixteen indigenous plants used by herbal doctors in Kenya were chosen from seven provinces and screened for antimicrobial activities using the disc diffusion method for preliminary selection. The most active plants, Ewntada abssyinica (Stem bark), Terminalia spinosa (Stem bark), Harrizonia abyssinica (roots), Ximenia caffra (roots), Azadirachta indica (stem bark and laves), and Spilanthes mauritiana (flowers and roots), were chosen for subsequent analysis, to determine efficacy and to identify a source of new possible antimicrobial agents. Thes plants were soxhlet extracted with methanol and tested for bioactivity. In the bioassay, hte microtitre broth dilution method and the agar dilution method were compared to determine suitability for use. The microtitre method was found superior with a mean of 0.856 mg/ml compared to 2.958 mg/ml for the agar dilution method. Subsequently 110 strains of pathogenic bacteria from six genera were tested for activity using the microtitre broth dilution method. Their minimum inhibitory concentration (MICs) in mg/ml and minimum bactericidal concentrations (MBC's) in mg/ml were determined as a measure of their efficacy. The extracts had a bacteriostatic effect on Gram negative bacteria and bactericidal effect on Gram positive bacteria. Bacteria with special significance and some protozoa were examined separately. Mycobacteria species whic were tested because of tuberculosis upsurgence in Human Immunodeficiency virus victims were found to be resistant to the extracts.......